March 19, 2008

Mark Blaxill and Boyd Haley Respond to Pichichero et. al. Thimerosal Mercury Excretion Study

Comment from Boyd Haley:

...Attached is a letter Mark Blaxill and I prepared and sent to the Pediatrics Journal in response to a Pichichero et al. study which claimed thimerosal (or ethyl mercury) left the body to fast to be toxic. Pediatrics refused to publish it which showed a total lack of support for any scientific debate in this journal for items concerning mercury toxicity causing autism. This same Pediatrics journal rejected the Nataf paper showing abnormal urinary porphyrin profiles in autistic children indicating they were mercury toxic. What the paper shows is that using Pichichero’s own data on fecal mercury excretion there was a definite retention of mercury in children receiving the normal vaccine schedule. Boyd Haley

From The Lancet 2002:

Mercury concentrations and metabolism in infants receiving vaccines containing thiomersal: a descriptive study.

Pichichero ME, Cernichiari E, Lopreiato J, Treanor J.

Department of Microbiology/Immunology, University of Rochester, Rochester, New York, NY, USA.

BACKGROUND: Thiomersal is a preservative containing small amounts of ethylmercury that is used in routine vaccines for infants and children. The effect of vaccines containing thiomersal on concentrations of mercury in infants' blood has not been extensively assessed, and the metabolism of ethylmercury in infants is unknown. We aimed to measure concentrations of mercury in blood, urine, and stools of infants who received such vaccines. METHODS: 40 full-term infants aged 6 months and younger were given vaccines that contained thiomersal (diptheria-tetanus-acellular pertussis vaccine, hepatitis B vaccine, and in some children Haemophilus influenzae type b vaccine). 21 control infants received thiomersal-free vaccines. We obtained samples of blood, urine, and stools 3-28 days after vaccination. Total mercury (organic and inorganic) in the samples was measured by cold vapour atomic absorption. FINDINGS: Mean mercury doses in infants exposed to thiomersal were 45.6 microg (range 37.5-62.5) for 2-month-olds and 111.3 microg (range 87.5-175.0) for 6-month-olds. Blood mercury in thiomersal-exposed 2-month-olds ranged from less than 3.75 to 20.55 nmol/L (parts per billion); in 6-month-olds all values were lower than 7.50 nmol/L. Only one of 15 blood samples from controls contained quantifiable mercury. Concentrations of mercury were low in urine after vaccination but were high in stools of thiomersal-exposed 2-month-olds (mean 82 ng/g dry weight) and in 6-month-olds (mean 58 ng/g dry weight). Estimated blood half-life of ethylmercury was 7 days (95% CI 4-10 days). INTERPRETATION: Administration of vaccines containing thiomersal does not seem to raise blood concentrations of mercury above safe values in infants. Ethylmercury seems to be eliminated from blood rapidly via the stools after parenteral administration of thiomersal in vaccines.

Blaxil and Haley's submission to Pediatrics:

Infant stool eliminates vaccinal mercury slowly suggesting high retention in tissue

To the editor,

In a study in The Lancet, Pichichero et al 1 argued that ethylmercury administered to infants through vaccines is eliminated rapidly from the blood and effectively excreted in stool. Our analysis of this data, combined with a more recent analysis2 of mercury excretion in baby hair suggests a more worrisome interpretation, one that offers support for the hypothesis3 linking early mercury exposures with autism.

Our calculations suggest that Pichichero et al. overstated the significance of their excretion findings. Although their data support a rapid rate of ethylmercury elimination from blood, instead of similarly rapid stool elimination, their findings demonstrate slow stool excretion in many infants, suggesting that significant amounts of ethylmercury from vaccines may be retained in infant tissue.

Most methyl mercury is eliminated from the body through stool and ethyl mercury from vaccines most likely follows the same path. Both mercury species must pass out of the blood to allow excretion in feces or (in lesser amounts) hair. 4 Nevertheless elimination from blood also allows for mercury transport into tissue, without prompt excretion. Our analysis of mercury excretion in autistic and control baby hair demonstrated that, although mercury was excreted at high rates in hair of normal infants, hair of autistic infants contained very little mercury, only 0.47 mcg/g versus 3.63 mcg/g in controls. This finding raises the possibility of increased mercury retention in the tissue of autistic infants, who also had higher rates of prenatal mercury exposure.

Pichichero et al provide data specific to infant mercury excretion through feces. They measured mercury concentrations in stool of 22 normal infants exposed to thimerosal in vaccines, ages two and six months, and found a range of 23-141 nanograms of mercury per gram of stool (dry weight). The authors interpreted these levels, mere parts per billion, as positive evidence of mercury elimination.

But these mercury concentrations are extremely low, not nearly enough to allow rapid excretion. Infant dry weight stool volumes have been measured at between 1-3 grams per kilogram (kg) per day.5 Based on the 50th percentile weight progression from 3.5-8 kg in the zero-six month period, infant stool volumes may be expected to range from 6-18 grams (dry weight) per day. Taking the stool concentration range for mercury from Pichichero et al, we calculated the time required for an infant to excrete the ethylmercury (187.5 mcg) that U.S. infants received by six months of age during the 1990s.

Stool Hg concentration Daily Hg excretion Days to excrete 187.5 mcg
(ng/g) (mcg/day) (days)

Minimum: 23 0.14-0.41 457-1,339
Maximum: 140 0.84-2.52 74-223

In the case of maximum excretion, early vaccine exposures are eliminated within the time period of exposure, but for those children with stool concentrations at the low end of the range, the infant elimination rate rises to nearly four years. For autistic infants, with evidence of reduced excretion in hair and additional fetal exposures2 (from maternal amalgam filling, fish consumption and Rho D immunoglobulin injections) these excretion times were likely far longer.

Our analysis contradicts the optimism expressed by Pichichero et al and suggests that low mercury excretion rates in some infants may underlie the link between mercury exposures and autism.

Mark F. Blaxill
Director, Safe Minds

Boyd E. Haley, PhD
Professor of Chemistry and Department Chairman
University of Kentucky

1. Pichichero ME, Cernichiari E, Lopreiato J, Treanor J. Mercury concentrations and metabolism in infants receiving vaccines containing thiomersal: a descriptive study. Lancet. 2002;360(9347):1737-1741
2. Holmes AS, Blaxill MF, Haley BE. Reduced levels of mercury in first baby haircuts of autistic children. Int J Toxic. 2003;111(4):277-285
3. Bernard S, Enayati A, Redwood L, Roger H, Binstock T. Autism: a novel form of mercury poisoning. Med Hypotheses. 2001;56:462-471
4. Clarkson TW. The three modern faces of mercury. Environ Health Perspect. 2002;110 Suppl 1:11-23
5. Chou C, Studies on the use of soybean food in infant feeding in China and the development of formula 5410. Food Nutr Bull. 1983;5(1) :10-11 - Contents

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