Showing posts with label Hepatitis B Vaccine. Show all posts
Showing posts with label Hepatitis B Vaccine. Show all posts

April 6, 2012

Hepatitis B Vaccine Causes Mitochondrial Disorders and Cell Death


Apoptosis. 2012 Jan 17. [Epub ahead of print]

Hepatitis B vaccine induces apoptotic death in Hepa1-6 cells.

Source

Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China, Heyam68_hamza@yahoo.com.

Abstract

Vaccines can have adverse side-effects, and these are predominantly associated with the inclusion of chemical additives such as aluminum hydroxide adjuvant. The objective of this study was to establish an in vitro model system amenable to mechanistic investigations of cytotoxicity induced by hepatitis B vaccine, and to investigate the mechanisms of vaccine-induced cell death. The mouse liver hepatoma cell line Hepa1-6 was treated with two doses of adjuvanted (aluminium hydroxide) hepatitis B vaccine (0.5 and 1 μg protein per ml) and cell integrity was measured after 24, 48 and 72 h. Hepatitis B vaccine exposure increased cell apoptosis as detected by flow cytometry and TUNEL assay. Vaccine exposure was accompanied by significant increases in the levels of activated caspase 3, a key effector caspase in the apoptosis cascade. Early transcriptional events were detected by qRT-PCR. We report that hepatitis B vaccine exposure resulted in significant upregulation of the key genes encoding caspase 7, caspase 9, Inhibitor caspase-activated DNase (ICAD), Rho-associated coiled-coil containing protein kinase 1 (ROCK-1), and Apoptotic protease activating factor 1 (Apaf-1). Upregulation of cleaved caspase 3,7 were detected by western blot in addition to Apaf-1 and caspase 9 expressions argues that cell death takes place via the intrinsic apoptotic pathway in which release of cytochrome c from the mitochondria triggers the assembly of a caspase activation complex. We conclude that exposure of Hepa1-6 cells to a low dose of adjuvanted hepatitis B vaccine leads to loss of mitochondrial integrity, apoptosis induction, and cell death, apoptosis effect was observed also in C2C12 mouse myoblast cell line after treated with low dose of vaccine (0.3, 0.1, 0.05 μg/ml). In addition In vivo apoptotic effect of hepatitis B vaccine was observed in mouse liver.

January 19, 2012

Hepatitis B Vaccine Can Cause Mitochondrial Disorders and Cell Death


Apoptosis. 2012 Jan 17. [Epub ahead of print]

Hepatitis B vaccine induces apoptotic death in Hepa1-6 cells.

Source

Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China, Heyam68_hamza@yahoo.com.

Abstract

Vaccines can have adverse side-effects, and these are predominantly associated with the inclusion of chemical additives such as aluminum hydroxide adjuvant. The objective of this study was to establish an in vitro model system amenable to mechanistic investigations of cytotoxicity induced by hepatitis B vaccine, and to investigate the mechanisms of vaccine-induced cell death. The mouse liver hepatoma cell line Hepa1-6 was treated with two doses of adjuvanted (aluminium hydroxide) hepatitis B vaccine (0.5 and 1 μg protein per ml) and cell integrity was measured after 24, 48 and 72 h. Hepatitis B vaccine exposure increased cell apoptosis as detected by flow cytometry and TUNEL assay. Vaccine exposure was accompanied by significant increases in the levels of activated caspase 3, a key effector caspase in the apoptosis cascade. Early transcriptional events were detected by qRT-PCR. We report that hepatitis B vaccine exposure resulted in significant upregulation of the key genes encoding caspase 7, caspase 9, Inhibitor caspase-activated DNase (ICAD), Rho-associated coiled-coil containing protein kinase 1 (ROCK-1), and Apoptotic protease activating factor 1 (Apaf-1). Upregulation of cleaved caspase 3,7 were detected by western blot in addition to Apaf-1 and caspase 9 expressions argues that cell death takes place via the intrinsic apoptotic pathway in which release of cytochrome c from the mitochondria triggers the assembly of a caspase activation complex. We conclude that exposure of Hepa1-6 cells to a low dose of adjuvanted hepatitis B vaccine leads to loss of mitochondrial integrity, apoptosis induction, and cell death, apoptosis effect was observed also in C2C12 mouse myoblast cell line after treated with low dose of vaccine (0.3, 0.1, 0.05 μg/ml). In addition In vivo apoptotic effect of hepatitis B vaccine was observed in mouse liver.
PMID:
22249285
[PubMed - as supplied by publisher]  

July 16, 2010

Film: Shots in the Dark

Made a film about the vaccine injured, including our community. It was made by the Film Board of Canada, but has not been released, and it is reported that the Canadian Prime Minister does not want it released.

It was posted on You Tube last fall. I am not sure how I missed this until now.

Shots in the Dark

February 2, 2009

May Ian Gromowski Rest In Peace

Today the Age of Autism has the story of Ian Gromowski, who was killed by the birth dose of the Hep B vaccine.

It is an important story to read, but a note of caution, the pictures of what happened to Ian after he got the shot are very difficult to look at.

November 23, 2008

Hep B Vaccine is Associated with a 9 Fold Developmental Disability Rate

Boys getting the recommended 3 Hep B shots, while the vaccines still contained Thimerosal, were NINE TIMES more likely to have a developmental disability than unvaccinated boys.

Finally... someone actually did a vaccinated v. unvaccinated study.

After Chandler was diagnosed, I printed out the Engerix B vaccine package insert, which I believed to be most significantly responsible for his autistic regression at 18 months and took it into my pediatrician.  My main question, "If the vaccine is known to cause Gilliam-Barre, a disorder in which the immune system attacks the central nervous system, then why couldn't it cause autism, a disorder in which the immune system attacks the central nervous system?"

He wouldn't take the highlighted print out from me and he didn't answer the question. He did remind me that vaccines were not associated with autism.

I will be sending this to him this week, as I am sure the American Academy of Pediatrics International Pediatricians Union won't be bringing this to his attention:

Hepatitis B triple series vaccine and developmental disability in US children aged 1-9 years

Authors: Carolyn Gallagher a; Melody Goodman a
Affiliation:   a Graduate Program in Public Health, Stony Brook University Medical Center, Health Sciences Center, New York, USA
DOI: 10.1080/02772240701806501
Publication Frequency: 8 issues per year
Published in: journal Toxicological & Environmental Chemistry, Volume 90, Issue 5 September 2008 , pages 997 - 1008

Abstract

This study investigated the association between vaccination with the Hepatitis B triple series vaccine prior to 2000 and developmental disability in children aged 1-9 years (n = 1824), proxied by parental report that their child receives early intervention or special education services (EIS). National Health and Nutrition Examination Survey 1999-2000 data were analyzed and adjusted for survey design by Taylor Linearization using SAS version 9.1 software, with SAS callable SUDAAN version 9.0.1. The odds of receiving EIS were approximately nine times as great for vaccinated boys (n = 46) as for unvaccinated boys (n = 7), after adjustment for confounders. This study found statistically significant evidence to suggest that boys in United States who were vaccinated with the triple series Hepatitis B vaccine, during the time period in which vaccines were manufactured with thimerosal, were more susceptible to developmental disability than were unvaccinated boys.
Keywords: early intervention; special education services; developmental disability; Hepatitis B vaccine triple series

Even though he was born in 2002, His first Hep B Shot contained thimerosal, which is known to cause mitochondrial dysfunction, which HHS has conceded become autism when multiple vaccines are later administered.

I would like to see this study repeated with children born since 2003 who received, "Thimerosal Free" Hep B shots. Was it the thimerosal or the aluminum or the shot itself.

August 2, 2008

CDC Reiterates that They Want ALL Newborns to Get Hep B Shot Before Leaving Hopsital

From Reuters:
"... Delivery hospitals play a key role in the national strategy to prevent hepatitis B transmission and should have policies and procedures in place to ensure that hepatitis B vaccination is administered to all newborns before they leave the hospital, they emphasize."
And by ALL they mean babies who weight 4 and a half pounds and are "medically stable".

So they remain bullish on their one size fits all vaccine schedule, even for babies who have no medical history yet, even for a disease that the vast majority of them are not at risk for, even for a vaccine which many adult health professionals (who are required to get it because they come into contact with blood products of high risk individuals) report multiple health problems after receiving.

Note the rational given for this.  Not the good of the individual infant, but as a key in their "national strategy to prevent hepatitis B transmission".

So your baby is drafted into the CDC's war on Hepatitis B, whether or not you want him to be, and whether or not it is in his best interest.

Mine was, and after his first Hep B shot he was left with three months of fevers and crying, and two years of bowel problems (until we put him on GFCF).  And even though all this was reported to his doctors, they continued giving him the Hep B shot (contraindicated for any one who has reacted to it before) until he regressed into autism at 18 months following the FOURTH time they gave him the shot.
CONTRAINDICATIONS
Hypersensitivity to any component of the vaccine, including yeast, is a contraindication (see DESCRIPTION). This vaccine is contraindicated in patients with previous hypersensitivity to any hepatitis B-containing vaccine.
Yes there is yeast in the vaccine.  Also some combination of:
Aluminum Hydroxide, Phosphate Buffers, Thimerosal, Aluminum Hydroxyphosphate Sulfate, Amino Acids, Dextrose, Formaldehyde or Formalin, Mineral Salts, Potassium Aluminum Sulfate, Soy Peptone, Aluminum Phosphate, Amino Acids, Inorganic Salts, MRC-5 Cellular Protein, Neomycin Sulfate, 2-Phenoxyethanol, and Polysorbate 20.
Depending on what brand of the shot.

The Vaccine Injury Compensation Fund even pays for "anaphylactic shock and death" as a result of getting a Hep B vaccine".

CDC knows full well that some children will get very sick and a few will die from this shot, yet they want ALL children to have it with in 48 hours of birth.

Known side effects:
 ADVERSE REACTIONS
ENGERIX-B is generally well tolerated. As with any vaccine, however, it is possible that expanded commercial use of the vaccine could reveal rare adverse reactions.
Ten double-blind studies involving 2,252 subjects showed no significant difference in the frequency or severity of adverse experiences between ENGERIX-B and plasma-derived vaccines. In 36 clinical studies, a total of 13,495 doses of ENGERIX-B were administered to 5,071 healthy adults and children who were initially seronegative for hepatitis B markers, and healthy neonates. All subjects were monitored for 4 days post-administration. Frequency of adverse experiences tended to decrease with successive doses of ENGERIX-B. Using a symptom checklist,† the most frequently reported adverse reactions were injection site soreness (22%) and fatigue† (14%). Other reactions are listed below.
Incidence 1% to 10% of Injections:
Local Reactions at Injection Site: Induration; erythema; swelling.
Body as a Whole: Fever (>37.5°C).
Nervous System: Headache†; dizziness.†
†Parent or guardian completed forms for children and neonates. Neonatal checklist did not include headache, fatigue, or dizziness.
Incidence <1% of Injections:
Local Reactions at Injection Site: Pain; pruritus; ecchymosis.
Body as a Whole: Sweating; malaise; chills; weakness; flushing; tingling.
Cardiovascular System: Hypotension.
Respiratory System: Influenza-like symptoms; upper respiratory tract illnesses.
Gastrointestinal System: Nausea; anorexia; abdominal pain/cramps; vomiting; constipation; diarrhea.
Lymphatic System: Lymphadenopathy.
Musculoskeletal System: Pain/stiffness in arm, shoulder, or neck; arthralgia; myalgia; back pain.
Skin and Appendages: Rash; urticaria; petechiae; pruritus; erythema.
Nervous System: Somnolence; insomnia; irritability; agitation.
Postmarketing Reports: Additional adverse experiences have been reported with the commercial use of ENGERIX-B. Those listed below are to serve as alerting information to physicians. 8
Hypersensitivity: Anaphylaxis; erythema multiforme including Stevens-Johnson syndrome; angioedema; arthritis. An apparent hypersensitivity syndrome (serum sickness–like) of delayed onset has been reported days to weeks after vaccination, including: arthralgia/arthritis (usually transient), fever, and dermatologic reactions such as urticaria, erythema multiforme, ecchymoses, and erythema nodosum (see CONTRAINDICATIONS).
Cardiovascular System: Tachycardia/palpitations.
Respiratory System: Bronchospasm including asthma-like symptoms.
Gastrointestinal System: Abnormal liver function tests; dyspepsia.
Nervous System: Migraine; syncope; paresis; neuropathy including hypoesthesia, paresthesia, Guillain-Barré syndrome and Bell’s palsy, transverse myelitis; optic neuritis; multiple sclerosis; seizures.
Hematologic: Thrombocytopenia.
Skin and Appendages: Eczema; purpura; herpes zoster; erythema nodosum; alopecia.
Special Senses: Conjunctivitis; keratitis; visual disturbances; vertigo; tinnitus; earache.
Note that in their safety study they only follow children for 4 DAYS to see if they will have adverse reactions to the shot!
 "Carcinogenesis, Mutagenesis, Impairment of Fertility: ENGERIX-B has not been evaluated for carcinogenic or mutagenic potential, or for impairment of fertility."
Does it cause cancer, alter genes, make people infertile?  They can't tell you, they've never checked to find out.

But they want EVERYONE to have it the day they are born.


How in the hell are then not in jail for this?

July 14, 2008

March 21, 2008

Serious Concerns Over Hepatitis B Vaccine

Today is one of those days that I read things like this and it just makes me cry.

If I knew this in March of 2002, would I have allowed Chandler to have that first Hep B shot that sent him on his journey down the rabbit hole?

All this information was out there. My doctor told me none of it.

Serious Concerns Over Hepatitis B Vaccine
Friday, March 21, 2008 7:05 AM
Michael Arnold Glueck and Robert J. Cihak, The Medicine Men


As some readers know, we have expressed our concerns about childhood vaccinations a number of times. (See Newsmax,com Medicine Men Archives.)

We are not saying that all vaccines are bad, but we ask that parents, physicians, and health authorities proceed with care and caution and sometimes resist some of the "automatic" childhood vaccinations.

Today the issue is that of the hepatitis B vaccine.

From 1994 to 1998, almost two-thirds of the French population and almost all newborn babies were vaccinated against hepatitis B, but the campaign was temporarily suspended because of concerns about side effects.

In what was called a "thunderclap in the vaccine industry," French authorities have opened a formal investigation regarding a hepatitis B vaccination campaign by GlaxoSmithKline and Sanofi Pasteur in the 1990s. It is alleged that the companies failed to fully disclose neurologic side effects.

Another investigation opened by Judge Marie-Odile Bertella-Geffroy concerns the death ("manslaughter") of a 28-year-old woman from multiple sclerosis, allegedly connected to the vaccine (Le Figaro 1/31/08).

Some 30 plaintiffs, including the families of five patients who died after the vaccination, have launched civil actions (Reuters 1/1/08).

A British case-controlled analysis showed an odds ratio of 3-to-1 (95 percent) for the first symptoms of multiple sclerosis in recipients of recombinant hepatitis B vaccine compared to controls. Two previous French studies had shown a ratio of about 1-to-5. Other studies showed a non-significant increase (or null findings) especially when date of diagnosis rather than date of first symptoms was used (Neurology 2004; 63: 838-842).

According to attorney Clifford Miller, "British doctors administering hepatitis B vaccine to infants could face criminal prosecution if fully informed consent is not obtained. Civil prosecution for damages is possible over 21 years later if the injured survive as adults" (UK Press Association Newswire/Romeike, September 2005).

The hepatitis B vaccine has been considered "one of the safest vaccines ever produced" (Neurology, 2004; 63: 838-842). On the other hand, French medical expert Marc Girard has said that "for a preventive measure, hepatitis B is remarkable for the frequency, variety and severity of complications from its use" (Romeike, September 2005).

In the past, individual concerns over vaccination have often been transgressed because of the platitude that the public good takes precedence over the individual.

We suggest that when it comes to the routine childhood hepatitis B vaccination those affected and involved should think a little harder before they shoot.

We think that the French authorities finally got something right.

* * *

Michael Arnold Glueck, M.D., comments on medical-legal issues and is a visiting fellow in Economics and Citizenship at the International Trade Education Foundation of the Washington International Trade Council.

Robert J. Cihak, M.D., is a senior fellow and board member of the Discovery Institute and a past president of the Association of American Physicians and Surgeons.

February 21, 2008

New Study Implicates Mercury In The Development Of Autism

The American Journal of Biochemistry and Biotechnology has published this study out of Rutgers and UMDNJ that exposed mercury to animals and found "neurobehavioral alterations" such as impaired social interaction, cognition and motor behavior. They also found that they improved when given vitamin E, suggesting that oxidative stress is at work in this process.

This is no surprise to any of us who have been treating our autistic kids for mercury toxicity and oxidative stress for years, but the big surprise in this study is in the credits.

The shocker is that this study is brought to you by Autism Speaks.

IMHO Autism Speaks has finally said something worth saying.

So... will we see this article in the press? Will the AAP recognize it and start looking at Vit. E as a helper for their patients with autism? Will CDC start taking another look at vaccines? Will Autism Speaks start coming around now that their own studies are implicating mercury as a factor in the development of autism?

Or will they all continue to proffer the lie that there is no convincing evidence linking vaccines to autism, while ignoring all the studies that are piling up on the hard drives of parents across the country?

American Journal of Biochemistry and Biotechnology 4 (2): 218-225, 2008
ISSN 1553-3468
© 2008 Science Publications

Corresponding Author: George C. Wagner, Psychology, Busch Campus, Rutgers University, New Brunswick, NJ 08854
Tel: 732-445-4660 Fax: 732-445-2263


Evidence of Oxidative Stress in Autism Derived from Animal Models
1Xue Ming, 2Michelle A. Cheh and 2Carrie L. Yochum,
3Alycia K. Halladay and 2George C. Wagner
1Pediatric Neuroscience, UMDNJ, Newark, NJ
2Psychology, Rutgers University, New Brunswick, NJ
3Autism Speaks, Princeton, NJ

Abstract: Autism is a pervasive neurodevelopmental disorder that leads to deficits in social interaction, communication and restricted, repetitive motor movements. Autism is a highly heritable disorder, however, there is mounting evidence to suggest that toxicant-induced oxidative stress may play a role. The focus of this article will be to review our animal model of autism and discuss our evidence that oxidative stress may be a common underlying mechanism of neurodevelopmental damage. We have shown that mice exposed to either methylmercury (MeHg) or valproic acid (VPA) in early postnatal life display aberrant social, cognitive and motor behavior. Interestingly, early exposure to both compounds has been clinically implicated in the development of autism. We recently found that Trolox, a water-soluble vitamin E derivative, is capable of attenuating a number of neurobehavioral alterations observed in mice postnatally exposed to MeHg. In addition, a number of other investigators have shown that oxidative stress plays a role in neural injury following MeHg exposure both in vitro and in vivo. New data presented here will show that VPA-induced neurobehavioral deficits are attenuated by vitamin E as well and that the level of glial fibrillary acidic protein (GFAP), a marker of astrocytic neural injury, is altered following VPA exposure. Collectively, these data indicate that vitamin E and its derivative are capable of protecting against neurobehavioral deficits induced by both MeHg and VPA. This antioxidant protection suggests that oxidative stress may be a common mechanism of injury leading to aberrant behavior in both our animal model as well as in the human disease state.

July 11, 2007

World Health Org Bans a Hep B Vaccine After Infant Deaths

WHO bans vaccine, sends experts to probe child deaths in Vietnam
Thanh Nien News

The World Health Organization has suspended the use of a Hepatitis B vaccine worldwide after three newborns died and another became seriously ill in Vietnam after getting shots in the last few weeks.

Nguyen Tran Hien, head of a council set up in Vietnam to investigate the cases, told the council’s first meeting that two international experts from the UN health agency would arrive in Vietnam Saturday to work with local investigators.

The cases occurred between April 23 and May 7 in Ho Chi Minh City, Ha Tinh Province, and Thanh Hoa Province.

One baby fully recovered after taking ill.

The WHO-approved vaccine is made by South Korea's LG Life Sciences and distributed in Vietnam through a UN program.

Earlier this month, Vietnam instructed all localities nationwide to stop using the vaccine.

WHO's Department of Immunization, Vaccines and Biologicals has asked that countries around the world stop using shots from the affected batches until the cause of the illness can be determined.